Problems in Static Culturing
Current procedures typically require multiple, stressful manipulations of the embryo for washing and culture media exchange. These manipulations produce detrimental variations in temperature, pH, humidity, and osmolality. Additionally, daily inspection and monitoring of embryo health requires regular personnel handling and undesirable drops in temperature and CO2 levels when out of the incubator.1,2
Dynamic Culturing with Microfluidics
Our dynamic culture system provides sustained, fresh media exchange through microfluidic technology, without any disruption or handling of embryos.3,4 The integrated environmental control and imaging capabilities allow regular monitoring of embryos, while maintaining the optimal culturing environment. Automation features substantially reduce the possibility of human error in the production process.
- Wheeler, M.B., Beebe, D.J., Walters, E.M., and Raty, S. (2002). Microfluidic technology for in vitro embryo production. Proceedings of the 2nd annual international IEEE-EMBS special topic conference on microtechnologies in medicine and biology. 104-108.
- Smith, G.D. and Takayama, S. (2007). "Gamete and embryo isolation and culture with microfluidics", Theriogenology 68S S190-S195.
- Wheeler, M.B., Walters, E.M., and Beebe, D.J. (2007). "Toward culture of single gametes: The development of microfluidic platforms for assisted reproduction", Theriogenology 68S S178-S189.
- Hickman, D.L., Beebe, D.J., Rodriguez-Zas, S.L., and Wheeler, M.B. (2002). "Comparison of static and dynamic medium environments for culturing of pre-implantation mouse embryos." Comp Med 52:122-126.