Conventional methods of live cell observation are currently very limited. In order to minimize cell degradation, it is critical to maintain environmental conditions without disruption to cells, restricting the opportunity and time available for cell analysis. Nanopoint's live cell imaging system and integrated microfluidic delivery platform enables researchers to considerably extend experiment time and automate the continuous capture of critical cell response data, while maintaining environmental conditions on-microscope and significantly reducing the amount of reagents and numbers of cells required for experiments.
Stem cell culturing, proliferation, and differentiation performed in well plates have the limitations of necessitating dedicated personnel, periodic disruption of incubation, and potential well-to-well variability. The cellTRAY Live Cell Imaging System can be effectively used to mitigate these issues for real-time monitoring of stem cells.
Drug-induced apoptosis experiments performed in 96-well plates have significant requirements; the attention of personnel during the entire time-course, potential well-to-well variability, and expensive use of reagents and cells. The cellTRAY Live Cell Imaging System allows real-time monitoring, where automated precise control of the microenvironment and data capture are required.
Cardiac Myocyte Cells in the cellTRAY
See a video of cultured cardiac cells on a Cell Matrix Chip Sensor for chem-bio toxins being developed by Cellular Bioengineering, Inc. in Honolulu, Hawaii.
View/Download Video (WMV, 7.9MB)
In vitro model systems have been proven useful in screening for inhibitory or stimulatory compounds that affect specific enzyme systems or affect the general process of cell death and apoptosis. A protocol has been developed for performing cell viability assays with the vital stain Trypan Blue, using Nanopoint's cellTRAY Live Cell Imaging System.