Assisted Reproductive Technology

Applications Overview
Assisted Reproductive Technology
Live Cell Imaging
Drug Discovery
CT-2000 Microfluidics System for ART
Assisted Reproductive Technology

Under current methodology used today by embryologists, embryos are cultured under static conditions that require extensive washings and nutrient media exchanges. This methodology has remained virtually unchanged for several decades. During this time, improvements in the percentage of live births from IVF were mainly realized with defined nutrient media formulations and timely-added growth factors during the process of oocyte maturation and fertilization. While these early in vitro manipulations resulted in increased embryo development, higher frequencies of successful implantations did not result, possibly due to a low quality status of in vitro-derived embryos.

However, some embryologists believe that embryo quality may be affected by the static culturing of the oocytes and zygotes. Experts in the IVF field have shown that moving fluid on the embryo itself results in increased live birth rates1, 2. These conditions more closely mimic actual embryonic conditions through natural conception in the female body, in which hormones and nutrients would be infused over time.

Nanopoint's microfluidics and imaging system for assisted reproduction applications is a research platform which provides a dynamic culturing environment, where multiple embryos can be cultured on the same slide. This technology allows precise delivery of nutrients, environmental control, and the automatic capture of data over extended periods of time.

To learn more about Nanopoint's ART platform, please see the CT-2000 Microfluidics System for ART, or contact us to request more information.

References

  1. G.D. Smith and S, Takayama, "Gamete and embryo isolation and culture with microfluidics", Theriogenology 68S (2007) S190-S195.
  2. M.B. Wheeler, E.M. Walters, D.J. Beebe, "Toward culture of single gametes: The development of microfluidic platforms for assisted reproduction", Theriogenology 68S (2007) S178-S189.
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